FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345182
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23770.6886222340678251No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18510.5362388537061608No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10190.2952065866702203No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5620.16281266114687323No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5350.15499070055796654No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5220.15122457138552994No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4510.1306557120591456No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3950.114432386393265No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGC200.006936080352.52034838
AGCCTTA350.00126695939.9176837
TCTTACA459.2967224E-538.808862
AATACTA654.9320624E-737.614745
TAATACT501.7280823E-434.9279754
TTACACA400.002440951834.927974
CATATAA450.004346708331.0470891
TTAGATA450.004346708331.0470894
GGAGTGC450.004346708331.0470896
TAGATAC605.035417E-429.1066445
AGTAATA500.00715716628.03538966
CTAGGAC500.007274253327.9423793
CTTTTTT1404.0199666E-1027.4434079
CTTACAC808.770394E-526.195983
TCTTTTT1358.212737E-925.8725728
ATACTTT959.413909E-625.77240420
TCTAGGA1508.967618E-1025.6138462
ATCTGGG2900.025.2926717
TGACTGA700.001236575724.95726813
GTACTAG700.00123909724.9485511
Produced by FastQC (version 0.11.5)