FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413764
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20900.5051188600264885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17340.41907947525642636No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT10620.2566680523196798No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8940.21606519658549317No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG7430.1795709631577421No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7270.175704024516391No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6930.16748677990351987No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA6900.16676172890826654No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA6800.1643448922574221No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6770.16361984126216877No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6060.14646030104117325No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5860.14162662773948434No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG5730.13848474009338657No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5360.12954244448526211No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5300.12809234249475546No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA5190.12543382217882657No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA4770.11528310824527992No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4710.11383300625477324No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4680.11310795525951992No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4580.11069111860867549No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4470.10803259829274658No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC4340.10489071064664882No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4320.10440734331647994No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG4300.10392397598631103No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACT352.1055861E-549.914692
CTAAGAC350.001265132339.931753
TAAGACA556.7644723E-638.116674
AGGATTA951.9099389E-1036.7792435
TAGTGTT450.00432208531.08496522
GTCCGCT450.00433739631.06251111
GTCCTAC450.004340463331.0580271
TATCGTT605.0186913E-429.12531313
AGCTTAT1103.1979653E-828.61229721
CGGCTAG500.00723313827.97646722
TTATCGT500.007258699327.9562612
GTATTAT500.0072638227.9522231
CCTAGGG500.0072638227.9522232
TATTGTA500.0072638227.9522232
CGGTCCA1900.027.58841510
CAGGATT1154.9652954E-827.3445684
TTCCTAG657.9199346E-426.9393945
CGTGTAT657.94677E-426.923838
CCAGTAG1606.548362E-1126.2431135
GCTTATG1207.438939E-826.2279422
Produced by FastQC (version 0.11.5)