FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605993
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43140.7118894112638264No Hit
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34890.5757492248260294No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12570.2074281386088618No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT11180.18449057992419055No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8370.13812040733143782No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT7730.12755922923202082No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7550.1245888978915598No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA7350.12128852973549199No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT7230.11930830884185131No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA6910.11402771979214281No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG6690.11039731482046823No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6340.10462167054734955No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG6060.10000115512885462No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA305.9244194E-446.589454
ATATAAG1600.037.125972
ATCTGGG4300.036.5673037
TATAAGA1850.033.9977043
TTCCGAT44700.033.3005142
CCGATCT44550.033.255774
TCCGATC45250.032.9729773
GATCTGG10150.032.0159036
TCTTTTT2850.031.8769918
CTTCCGA49000.030.3782221
ATCTTAC1052.0547304E-829.9503617
CGATCTG23800.029.9503615
ATAACTA755.6453042E-527.953675
ATAAGAA2250.027.9536694
CGATCTA10550.027.8211885
GAGTGTA4250.027.23116170
CTAGCTA657.9426647E-426.92913640
TACTGGC658.030442E-426.87852910
TCTAGGC1056.862556E-726.6225433
ACCGGTA2250.026.40591215
Produced by FastQC (version 0.11.5)