FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375826
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN129700.9427064178173693No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN59860.43508408766806267No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN59170.43006891859871815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46070.3348533898908728No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44550.32380548121637476No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC36170.262896616287234No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34520.25090382068662753No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC32610.23702125123380427No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC31880.23171534772565716No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC27890.20271458745509968No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26880.1953735428753345No Hit
GACGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25340.18418026698143516No Hit
GCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23050.1675357203599874No Hit
CGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20700.15045507208033573No Hit
CACGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19610.1425325586229654No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA15710.11418595083971374Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15110.10982493425767503No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC14950.1086619965024647Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14910.10837126206366213No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT14910.10837126206366213No Hit
TCTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14200.10321072577491631No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAT400.002442207234.933211
GCCAATA605.0403306E-429.111012
TTTCGGA3300.028.5817171
CATACTA755.6495206E-527.95508411
TGGTATA808.560266E-526.29949663
GATAATT808.7502856E-526.21473918
ACCGGTA2850.025.75146915
TACAATA959.525291E-625.7447418
ACGATAG4400.025.50811469
ATAGACA1101.0339791E-625.4059723
TTCGGAA3800.024.8209652
ATATAAG3000.024.4532472
GGTAATA3100.023.67783218
GGTATAC1403.2277785E-722.54242764
AACTCCA3750.022.3611479
AATACGC3300.022.24862521
CATACAT3150.022.21462631
CTCTAGG952.8175703E-422.063081
ATAAGAA3350.021.8984324
ACGCTTA3400.021.59707524
Produced by FastQC (version 0.11.5)