FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471451
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33850.7179961438198244No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14580.30925801408842063No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11240.238412899749921No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8830.1872941196433988No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8070.1711736744645785No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7640.16205289627129862No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6980.14805356230021782No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5100.10817667159471504No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCG404.6217436E-543.6978034
TTCTAAA350.001262153439.952282
CTAACCC901.772205E-731.0739973
ACTCTAA1151.4861089E-624.36200150
TTTAAGC1553.4724508E-822.5937943
GTCCTAG1553.53848E-822.5537071
ACCGGTA1403.3094693E-722.47600415
TGCTTTA1253.0747124E-622.40735240
ATCTAAC952.7994346E-422.0788921
TAAGTTA800.002645307721.90163862
GCTAGCA800.002668809821.86830128
GGTAATA1656.848131E-821.189518
CGCTTAA850.003765341420.6080758
AGCTAGC850.003795924820.5793227
ATACAAT850.003812692620.5636736
ATTCCCC1205.674427E-520.43114552
AGTAATA1408.142551E-620.02435366
ATGTTAT1408.286757E-619.98373622
AGGGACG1601.1959801E-619.6640135
GTGTGTA3600.019.46812270
Produced by FastQC (version 0.11.5)