FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605903
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68801.135495285549007No Hit
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41700.6882289739446743No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15270.2520205379408916No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11250.185673284337592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG22350.032.3571625
TTCCGAT55750.032.019222
CCGATCT55400.031.9062294
TCCGATC57200.031.2075423
CTTCCGA58850.030.5700021
GATCTGG11350.029.8575046
ATCTGGG4600.029.6199557
GATCTAG4850.028.8134926
AGGGTCC500.007270923427.9490855
ACTCACG500.007270923427.9490858
ATCATAC500.00727443227.9463233
ATCTGGT2800.027.4499957
ATCTAGC2150.025.9991517
ATATAAG959.525054E-625.7400362
CGATCTC15850.025.3481465
AGGTGTA6700.025.13584770
CGATCTA14950.024.5372415
TCTAGGC1001.4165837E-524.455458
CTATACA1001.4176689E-524.4530334
TCTGGGG2051.8189894E-1223.8589768
Produced by FastQC (version 0.11.5)