FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250976
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18620.7419036083131455No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7730.30799757745760553No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6920.27572357516256535No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6400.2550044625780951No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5020.20001912533469335No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4850.19324556929746273No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4280.17053423434910112No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA3980.1585809001657529No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3650.14543223256406987No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3640.1450337880912916No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3510.13985400994517405No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3480.13865867652683922No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG3240.12909600918016065No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3170.12630689787071273No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC3030.1207286752518169No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2940.11714267499681244No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2860.11395511921458626No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2820.11236134132347317No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2710.10797845212291216No Hit
GCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2600.10359556292235114No Hit
GACGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2590.10319711844957286No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2540.10120489608568149No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTATA200.007006332752.3818132
TCTGATG601.2286757E-534.9295238
TTAGCTT400.002438943134.9295235
GGGGTTA400.002438943134.9295236
TAGTGTA1206.366463E-1132.14885370
TATTCCC553.0080517E-431.77681226
CTGATGT553.020693E-431.7541149
GTGTTAG450.00434821531.0410751
GCCCAAC450.00434821531.0410751
ATGTTAT1301.6734703E-1029.57687822
ATATAAG605.0366094E-429.1010062
TTATCTA1352.6011548E-1028.48143825
CATGTTA1352.6011548E-1028.48143821
ACAAGAG500.007125777728.05718469
TGTCTAG500.007209361527.9902729
GGAGCGA500.0072683327.9436198
CTTACAT657.9981145E-426.8880727
ATAGTGT1455.7480065E-1026.6059569
ATCTATT808.756821E-526.1971447
TCTGTCC808.756821E-526.1971448
Produced by FastQC (version 0.11.5)