FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559939
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32870.5870282298607526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17680.3157486797669032No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14500.2589567792205937No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT12010.21448764954754No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10460.18680606280326964No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG9160.16358924811452677No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA8880.158588703412336No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA8370.14948056841905993No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8290.14805184136129113No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8070.14412284195242697No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC7630.1362648431346986No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT7370.13162148019695002No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT7300.13037134402140235No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG6770.12090602726368407No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC6750.12054884549924187No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6440.1150125281503878No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA6400.11429816462150341No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT5630.10054666669047879No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC400.002433466334.9544758
ATAATAC501.7242269E-434.9470373
TAGTGTA2650.033.06747470
CTATACC1600.032.7628444
GTATAAG450.004337919431.0640323
TATACCT1653.6379788E-1227.5369595
ATAAGAG658.022951E-426.8823363
ACTATAC1757.2759576E-1225.9606533
ATAGTGT3450.025.39965469
TCGGTTA700.001225635124.9994333
GTATTAA700.001236340824.9621681
TATAATA700.001236340824.9621682
ATCTGTG3100.023.6763177
GACTTTA750.001846485523.3005057
GTCTTAC3600.023.2980251
TATATAC1052.0613044E-523.2980233
TTATAGT750.001847629723.2980234
ACCTTTA1553.54612E-822.5512758
AACGGGT3900.022.41626422
CAGACAT3600.022.360633
Produced by FastQC (version 0.11.5)