FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658389
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42760.6494640706330148No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA42210.6411103466187922No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG39910.606176591650225No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT38340.5823305067368987No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG34540.5246138680931789No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC29270.44457000344780967No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC25420.3860939353482516No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG24880.3778920972251967No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC22710.3449328588418093No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA21930.33308575933073No Hit
GATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGA20080.30498686946470854No Hit
TCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGG19930.3027085810971933No Hit
GGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTG19790.30058217862084574No Hit
GCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATAC19220.2919246828242878No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA17940.27248328875482425No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAA17880.27157197340781813No Hit
CCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGT17840.27096442984314745No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG17780.27005311449614133No Hit
GGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATG17660.2682304838021291No Hit
CTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAA17420.26458522241410476No Hit
GTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGC17090.25957298800557116No Hit
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAA16780.254864525379373No Hit
CTACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAA16720.25395321003236687No Hit
GCAATAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCT16590.251978693447187No Hit
CTTCCGATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTT16360.24848531795033027No Hit
CATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGG16300.24757400260332416No Hit
ATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAG15960.24240988230362295No Hit
GGGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT15540.23603067487458024No Hit
GTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACC15540.23603067487458024No Hit
ACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAAC15320.23268918526889118No Hit
CCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTG15170.23041089690137595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14610.22190528699598566No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13570.20610915431454657No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTG13070.19851485975616237No Hit
CTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGAACTTGGAATT13040.1980592020826593No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGC12690.19274319589179043No Hit
TAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATT12570.1909205651977782No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG12000.18226306940122025No Hit
CTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGAC11950.18150363994538182No Hit
AGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACA11720.1780102644485251No Hit
GGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATT11350.17239048647532082No Hit
GTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCAT11300.1716310570194824No Hit
CAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACC11170.1696565404343025No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC10940.1661631649374458No Hit
GTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATC10220.1552273807733726No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10010.15203777705885124No Hit
GCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGT9970.1514302334941805No Hit
CGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCA9930.15082268992950976No Hit
TAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATG9840.14945571690900059No Hit
GTCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9790.14869628745316218No Hit
GACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAACAGGTCTGTGAT9730.14778497210615607No Hit
GTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACT9500.14429159660929938No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC9060.1376086173979213No Hit
TCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCC8990.1365454161597475No Hit
AGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTC8910.13533032903040604No Hit
GTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCA8880.13487467135690298No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA8800.13365958422756152No Hit
AGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATA8730.13259638298938772No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGA8700.1321407253158847No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT8500.129103007492531No Hit
ATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACA8210.12469831664866819No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT8050.12226814238998525No Hit
ACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGGGATTGTG8030.12196437060764988No Hit
TCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGGG8000.12150871293414685No Hit
GTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACT8000.12150871293414685No Hit
TTCCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7710.11710402209028402No Hit
GAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTG7640.11604082085211022No Hit
TTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACC7580.11512950550510413No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG7570.11497761961393645No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGACG7530.1143700760492657No Hit
CTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAA7510.11406630426693035No Hit
GGGTAAACCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGT7420.11269933124642117No Hit
GGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTG7370.11193990179058277No Hit
GACGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7350.1116361300082474No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT7230.1098134993142352No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCAC7120.10814275451139067No Hit
ATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGTCCTT6980.10601635203504312No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6970.10586446614387543No Hit
GTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTC6900.10480126490570166No Hit
GTCTAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6830.10373806366752786No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA6790.10313052010285713No Hit
CCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGC6690.1016116611911803No Hit
GAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATC6620.1005484599530065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23450.041.4414185
TCTAGGC1400.039.954388
GCAGAGG350.001262252939.954388
CCGATCT44800.039.7946624
TTCCGAT45050.039.729022
TCCGATC45050.039.6514243
GATCTGG13350.038.236
CTAGGCG556.745375E-638.138279
CTTCCGA47550.037.7872471
TCTGGTC1250.036.3584868
ATCTGGG9100.035.7251637
ACGGGTC601.2172539E-534.9921230
TCCAGGA400.002432992434.956882
CGATCTA10150.033.7514725
TCTGAGC1800.033.0178538
TCTGGGC6850.032.6634338
ATCTGGT2150.032.518037
TAGGCGG652.118479E-532.27084410
ACTCCTA552.9985863E-431.80518725
ATCTGAG2900.031.340657
Produced by FastQC (version 0.11.5)