FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501031
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33960.6778023715099465No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21040.419934095894266No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14800.2953909039560426No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8430.16825306218577293No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8220.16406170476477502No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8090.16146705493272873No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6750.1347222028177897No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6340.12653907642441287No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT5450.10877570449732651No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5020.10019340120671176No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTTG150.002231750969.9378922
GACTAAT459.168421E-538.905744
AGTATAA450.00434442731.0537152
AGGTGTA4200.030.0490570
TTAATAC703.5297846E-529.9446533
CTAACGC500.007168934728.0289353
GTATAAT658.031404E-426.8766231
CTTTAGA658.031404E-426.8766231
TATTGTA658.0370286E-426.8734055
GGTTAGC808.6251894E-526.26450247
TAGGGTG1207.51952E-826.201575
ATAGGGT1101.0307704E-625.4075834
TAGGTGT5100.024.74627769
ATTCATT851.3149671E-424.6839620
GGGGTTA901.9203672E-423.3434245
CAGACGT901.9423559E-423.30424514
TCTAGGC901.9470953E-423.2958688
AGTGAGG750.001849570223.2930766
TAAAGTG1253.1340442E-622.3586715
GGTTAAT1102.9106628E-522.2796834
Produced by FastQC (version 0.11.5)