FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533729
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35940.67337543959575No Hit
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34790.6518289244166984No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12290.23026667091351605No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7950.14895199623779107No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6280.11766270897777711No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG200.00699449652.41388710
TATAAGA602.628658E-740.7663573
CTCTGAG350.001253929240.0065134
TTCCGAT40350.032.9941182
ATCTTAC851.0779331E-732.8871427
CATATAA652.1248467E-532.25471
ATATAAG652.1248467E-532.25472
CCGATCT40950.032.1693734
GGTCCAA1206.730261E-1132.03071211
TCCGATC42300.031.6383273
GTATAGA450.004340363731.0600811
CGATCTA8700.030.9262035
TACTAAT1251.05501385E-1030.80849341
ATAAGAA802.4958445E-630.5747684
CTTCCGA43950.030.3710351
CCTAGGG703.5253466E-529.9507922
GTATTAA605.0274987E-429.1188281
CGATCTG19550.028.9549875
ATACTAA1352.564775E-1028.52638440
GATCTGG8550.028.1992866
Produced by FastQC (version 0.11.5)