FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735673
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65970.8967299330001236No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26080.3545053304933034No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23300.3167168021661798No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18300.24875182316056182No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16630.22605152017268543No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13100.17806824499471913No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAT10850.147484004442191No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC10560.14354203565986517No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC9420.12804602044658428No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9410.12791009048857305No Hit
GACGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9200.12505556137033708No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC8990.12220103225210113No Hit
GCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8370.11377337485540451No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC7760.1054816474167191No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7540.10249118834047193No Hit
CGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7490.10181153855041575No Hit
CACGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7370.1001803790542809No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACTA305.92149E-446.59571511
TTACTCG305.931011E-446.5805324
CGTAAAT450.004321819631.08914423
TGTTGAA952.7861824E-729.51546362
AATCTAG604.928655E-429.21995469
TCTAGGC605.035783E-429.1128333
GTGTAGG1004.4909393E-727.9483171
AATTAGT1653.6379788E-1227.58103231
ATTGTTG906.1018254E-627.2608160
CAAGTTA657.897045E-426.95683353
TAGGCCG1705.456968E-1226.7153035
CTATAGG808.773757E-526.2015481
TTAGTGA1757.2759576E-1226.00497233
CTTAAGG2050.025.5624873
ACAAGAG1801.0913936E-1125.3239669
AGAGTGA1251.1182783E-725.1534866
CAAGATA700.001212923925.04567170
ATGCACT700.00123917724.9538544
AAATCTA1151.4744292E-624.38831968
TTTCGGA1301.6339982E-724.1860431
Produced by FastQC (version 0.11.5)