FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958633
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72480.7560766216059743No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28830.3007407422861512No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26260.2739317340421204No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAC19710.20560527334235312No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18510.19308744848132706No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15450.16116699508571058No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13090.13654860619235934No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA11920.12434372695285892No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC11280.11766755369364502No Hit
GTATTTAGCTTTAGATGAAATTTACCACCCATTTAGAGCTGCATTCCCAA10420.10869644587657634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT1600.030.5620044
CTCTTAG906.272061E-627.1662241
GTATTAA658.053495E-426.8676971
ATTATAC1056.8951886E-726.6118133
CTAGAAG808.789665E-526.1960033
CTTATAG3200.024.0130021
CAAACGT750.00181784523.3656565
ACTTGTA750.001844535923.30718619
CTAGGCG750.001851932323.2911599
AGGGGTT1652.7175702E-923.2897035
GGGTATT3650.022.98215716
AGGGTGC1154.1733096E-521.2671710
GATATGT1003.900553E-421.0251354
ATAATAG2351.8189894E-1120.8081743
AGTCACC1355.92337E-620.77989269
GATCTGG19350.020.7621366
TATGAGG850.003793774620.58447827
CACTGTG850.003831082720.5497387
GCACAAT850.003831082720.5497386
ATATTAC2051.4279067E-920.51746757
Produced by FastQC (version 0.11.5)