FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102360
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86170.7816865633731268No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28470.2582640879567473No Hit
GATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGACCTGTCGGC26920.24420334554954826No Hit
GGTGTGCACCGGTCGTCTCGTCCCTTCTGCCGGCGATGCGCTCCTGGCCT21250.1927682426793425No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC19460.17653035306070614No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC19250.1746253492506985No Hit
GATCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17870.16210675278493414No Hit
GTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGC17670.16029246344206974No Hit
GACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTT16620.15076744439203163No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCT16480.14949744185202657No Hit
CCCTAACGTTGCCGTCAACCGCCGCGTCCCGGTTCAGGAATTTTAACCCG15910.144326717224863No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGG15040.13643455858340287No Hit
ACACTGATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAA14720.13353169563481984No Hit
GGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATT13730.12455096338764107No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCC13270.12037809789905295No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12880.11684023368046736No Hit
GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTATAGCC12650.1147538009361733No Hit
CTTCCGATCTGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTT12570.11402808519902755No Hit
ACCTATACCCGGCCGTCGGGGCAAGCGCCAGGCCCCGATGAGTAGGAGGG12460.11303022606045211No Hit
GATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAATCTTT12080.10958307630900975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11540.10468449508327587No Hit
CCTATAAGCAACATCCGCCGATCCCTGGTCGGCATCGTTTATGGTTGAGA11290.1024166334046954No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT11250.10205377553612249No Hit
CATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACC11050.1002394861932581No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA459.294805E-538.8215981
TCTGACC1750.035.943568
TCTACGT400.002437873534.945138
GTATTAT400.002439822334.9394381
GTCTAGG708.8160596E-734.9394381
CCTGGGT1450.033.734633
ATCTGGG9950.033.7140737
AGGGTTA652.1278383E-532.253545
CCTATAT652.1286285E-532.251791
CGGTATG553.0206676E-431.7683019
CTAGGCC1001.2812961E-831.4454943
CCTATCC450.004344683631.057283
TATACTG703.5302888E-529.9497175
CTTTAGG703.5315974E-529.948091
CTGAGGC1103.23289E-828.591479
GATCTTT3800.028.5047726
TATAGGT8250.028.3750573
ATTCGGC6950.028.2215947
CAGTCAC500.007150239328.04748267
GATTCGG7000.028.01848246
Produced by FastQC (version 0.11.5)