FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674187
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN59300.8795779212592352No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24250.3596924888791982No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21750.3226107889947448No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13810.20483931016172072No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11280.16731262987865386No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA9840.14595357074520868No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT8840.1311208907914273No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8320.12340789721546098No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7550.11198673365104934No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG7300.10827856366260401No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGT553.0221645E-431.762963
ATAGGAC703.529306E-529.9479353
TAGCGTC853.999792E-628.7811789
ATCTGGG7000.028.4555937
AGACTGG808.755301E-526.2091036
TAGACTG808.7605964E-526.2067725
GAACGAT959.396606E-625.78596531
CTATAGG959.514102E-625.7447151
GATCTGG16900.025.640466
TAGTGTA4050.025.10972670
GGGAACG1401.2025339E-824.97214716
ATCTGCG4250.024.6673877
ACGATTC1001.39761705E-524.49884633
CCGGGTT1001.4111722E-524.46835114
ATCTGTG4500.023.2969787
AGTGTAG2850.022.147670
CACGTTA952.7714786E-422.11406745
GGTAAGG952.8050484E-422.07474511
ATAGCGT952.806736E-422.0727838
GATTTAG952.8118037E-422.0668981
Produced by FastQC (version 0.11.5)