FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8574220
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG807590.9418815938942551No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA136170.15881327980854237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT115300.028.7132786
CGATCTT150450.026.8047355
TTCGGAA9900.026.4307752
ATCTTTT153750.021.1693387
ATCTGGG19200.020.349687
CCTTTTT9300.019.89796625
TTCCGAT519950.019.5462192
CCGATCT521800.019.381734
TCCGATC529350.019.1909663
AGGGGTG18950.019.1456435
CTTTTTT169000.019.1340089
GATCTGG51550.018.9485156
TAGGACT10050.018.7445964
AGGACTA9450.018.4579755
TAGACTG2651.0004442E-1018.4301135
TCTAGGC3650.018.1595068
CAGGGGT8650.018.148674
CGATCTG168550.018.0274895
GCGCTAA2358.28004E-917.81463613
AGGGGGG139800.017.57640560
Produced by FastQC (version 0.11.5)