FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969400
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT18550.19135547761501961No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC10110.10429131421497834No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT9790.10099030328037961TruSeq Adapter, Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4600.034.9992839
TCGTATG4900.032.85646440
GTATGCC4950.032.52458242
TGCTTGA5350.029.43864455
GCCGTCT5800.027.75804746
GCTTGAA6150.026.74741656
GTCTTCT5900.026.69436549
CGCTAAA1850.026.4859410
TATGCCG6150.026.17832243
ATGCCGT6300.025.55502944
CGTATGC6400.025.15573141
GATCTTT24550.025.0950166
ATCTTTT21450.024.9683847
CCGTCTT6600.024.92372747
TTATACT1151.495675E-624.3510914
GTGCATA1752.0008883E-1024.0069331
TCTTTTT21600.023.9846978
CGATCTT49000.023.5031535
GTCTAGG1502.4698238E-823.3400751
ACTGTCG2103.6379788E-1223.3364628
Produced by FastQC (version 0.11.5)