FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699888
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT16640.23775232608645955No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC14500.20717600530370575No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC12960.18517248474041562No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC12000.17145600438927372No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC10240.14630912374551358No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC9350.13359280341997576Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA8820.12602016322611617Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT8410.12016208307614933No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGT400.002413924335.0143661
TCCTATA552.9850725E-431.8312421
GCTAACA450.00430469831.1149832
AATATAG500.007204090728.0034872
TATGCGT906.17192E-627.22172267
CTCGTAT3800.025.78939
ACGATAG1401.1896191E-824.99954269
TAGACCC851.3072E-424.7089584
TTAGACA851.3072E-424.7089584
GTATTAA1001.3933084E-524.5100571
GATCTTT18000.023.7251786
TTTCGGA3100.023.719411
TCGTATG4150.023.61402540
TATAACC750.001830990823.3362394
CTTAATC901.9261982E-423.3362373
TCTAACG901.9271312E-423.334578
TACTCTA1052.0394402E-523.33290754
TACCATA750.001832513723.33290549
TCTTTTT15500.023.25938
ATCTTTT16550.022.8410917
Produced by FastQC (version 0.11.5)