FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531411
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT23750.44692337945582605No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT12460.23447011823240393No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT5920.11140153290014695Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAATA200.006945225452.5091062
GAGTCTA400.00241844234.9994818
TCCTACA1154.0017767E-1133.4840662
CTCGTAT5700.032.5433839
TAATAGT450.004306709431.11065168
TTTCGGA3000.030.335741
GTATGCC6150.030.16215742
TATGCTT604.978417E-429.1689825
TCGTATG6400.028.98394640
TGCTTGA6450.028.75926655
GCTTGAA6450.028.75926656
GCCGTCT6450.028.75926646
GTCTTCT6350.028.66099449
CTTGAAA6700.028.2085457
TCCATAG500.007200835728.0048562
ATAGACT500.007200835728.0048564
TACTAAT500.007200835728.0048562
GTATGTA500.00720414528.0022221
AAGTAAT500.007207455627.99958665
TCTTTTT14150.027.9527458