FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571872
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT26020.4549969223882267No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT14780.25844944323205193No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT8250.14426305187174754Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATC200.006950569352.4994114
TTCTAAT400.002417516435.0026661
ACTATAC501.7086453E-435.0026663
TTTCGGA4400.033.4116361
GGCGTAT552.990251E-431.8206061
ATTCTTA450.00430690631.1107642
TCGTATG6350.030.86579540
TATTAAT802.4625988E-630.6300132
CTCGTAT6150.030.16226239
GTATGCC6500.030.15350742
GATCTTT13600.030.1125896
TCTTTTT13100.029.6587478
ATCTTTT13400.029.5171747
TTAAGGT952.818524E-729.475934
ATCTAGC2050.029.0266047
CGATCTG32700.029.0083245
CTTTTTT13550.028.9295659
TCTTCTG6800.028.82320650
CGATCTT21050.028.7670385
GCCGTCT6950.028.20112246
Produced by FastQC (version 0.11.5)