FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7339
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAGCA1371.8667393377844392Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
GCAATGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCG1271.7304809919607576No Hit
GTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCAATGAG1031.4034609619839216TruSeq Adapter, Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT961.3080801199073444No Hit
GAAGAGCACACGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCG741.0083117590952446Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
CTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACG680.9265567516010355Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTG670.9129309170186675TruSeq Adapter, Index 2 (95% over 22bp)
GATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCC580.790298405777354Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCCCCACAGATCGGAAGAGCACACGTCTCCCCACAGATCGGAAGAGCA540.7357950674478811Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGG490.6676658945360403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCA440.5995367216241995TruSeq Adapter, Index 2 (95% over 22bp)
GTGGGGAGACGTGTGCTCTTCCGATCTGTGGGGAGACGTGTGCTCTTCCG420.5722850524594632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAGAG400.5450333832947268Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAG390.5314075487123586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACACGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATCGG340.4632783758005177Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
GAAGAGCACACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCT330.44965254121814957Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAGCACACGTCTCAT320.4360267066357814Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGA310.42240087205341326Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAGAGC300.40877503747104516Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCC290.395149202888677Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAG280.3815233683063088Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTGTGGGGAGACGTGTGCTCTTCCGATCTGTGGGGAGACGTG280.3815233683063088Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCG270.3678975337239406Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCACACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATC270.3678975337239406Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAG260.3542716991415724Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
AATGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGAT260.3542716991415724Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGC260.3542716991415724Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGCACACGTCTCCCGCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGA250.34064586455920426Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCACACGTCTCCCCACAGATCGGAAGAGCACACGTCTCCC250.34064586455920426Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATC220.29976836081209973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCT210.2861425262297316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTC200.2725166916473634Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAAG200.2725166916473634Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
GCCTAGATCGGAAGAGCACACGTCTCCCGCCTAGATCGGAAGAGCACACG190.2588908570649952Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCCCCACAGATCGGAAGAGCACACGTCTCCCCACAG190.2588908570649952Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGGGAGACGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGA180.24526502248262708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCACACGTCTCATTGCCGATCGGAAGAGCACACGTCTCATTGCCGATC170.23163918790025886Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
CGTGTGCTCTTCCGATCTAGGCGGGAGACGTGTGCTCTTCCGATCTAGGC170.23163918790025886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAATGAGACGTGTGCTCTTCCGATCGGAAGAGCACACGTCTCATTGCCG160.2180133533178907Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
TCTCATTGCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCAATGAG140.1907616841531544TruSeq Adapter, Index 5 (95% over 22bp)
CGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCAAT140.1907616841531544TruSeq Adapter, Index 2 (95% over 22bp)
GCACACGTCTCCCCACAGATCGGAAGAGCACACGTCTCCCCACAGATCGG120.16351001498841805Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACGTGTGCTCTTCCGATCTGTGGGGAGACGTGTGCTCTTCCGATCTGTG110.14988418040604987Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCGATCTGTGGGGAGACGTGTGCTCTTCCGATCTGTGGGGAGACGTGTG110.14988418040604987Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GACGTGTGCTCTTCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAA110.14988418040604987Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
GGGGAGACGTGTGCTCTTCCGATCTGTGGGGAGACGTGTGCTCTTCCGAT100.1362583458236817Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATC100.1362583458236817TruSeq Adapter, Index 2 (95% over 22bp)
CGGCAATGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTC100.1362583458236817Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTCTTCCGATCGGCAATGAGACGTGTGCTCTTCCGATCGGCAATGAGACG90.12263251124131354TruSeq Adapter, Index 1 (95% over 22bp)
CGTGTGCTCTTCCGATCTGTGGGGAGACGTGTGCTCTTCCGATCTGTGGG90.12263251124131354Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG90.12263251124131354No Hit
CGTCTCCCCACAGATCGGAAGAGCACACGTCTCCCCACAGATCGGAAGAG90.12263251124131354Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCGGAAGAGCACACGTCTCATTGCCGATCGGAAGAGCA90.12263251124131354Illumina Multiplexing Index Sequencing Primer (95% over 22bp)
GGCAATGAGACGTGTGCTCTTCCGATCGGCAATGAGACGTGTGCTCTTCC80.10900667665894535No Hit
TCCCCACAGATCGGAAGAGCACACGTCTCCCCACAGATCGGAAGAGCACA80.10900667665894535Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTCACGGGCTCTCAGGGTCTCAGGCTCCGAGATCGGAAGCTGTCTCTTA80.10900667665894535No Hit
GTGCTCTTCCGATCTGTGGGGAGACGTGTGCTCTTCCGATCTGTGGGGAG80.10900667665894535Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGCACACGTCTCCCCACAGATCGGAAGAGCACACGTCTCCCCACAGATC80.10900667665894535Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC2900.00321247879.6551723
CTTCCGA2900.00321247879.6551721
TTCCGAT2950.00370193889.4915262
Produced by FastQC (version 0.11.5)