FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897255
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT19990.2227906225097659No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA17910.1996088068609258No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA16160.18010487542560366No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG14120.15736886392385666No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA12620.1406512084078662No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT12590.1403168552975464No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT12380.13797638352530775No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA12380.13797638352530775No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC12370.1378649324885345No Hit
GAATAGGCCCAAGCGACTGTTTATCAAAAACACAGGTCTCTGCTAAACCG12250.13652752004725524No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGGTTA12120.13507865656920273No Hit
ATTCTAAGGTGAGCGAGCGAACTCTCGTTAAGGAACTCGGCAAAATGACC11660.12995190887763233No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC10970.12226178734027673No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT10830.12070147282545096No Hit
TTCCAGCACCGGGCAGGCGTCAGCCCCTATACATCACCTTACGGTTTAGC10200.11368005750873499No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA9880.11011362433199035No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT9820.10944491811135074No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA9820.10944491811135074No Hit
GTGCTGGAAGGTTAAGAGGAGTGGTTAGCTTCTGCGAAGCTACGAATCGA9670.10777315255975169No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG9580.10677009322879226No Hit
GGTACTATGATTTCACCGAGTCTCTCGTTGAGACAGTGCCCAAATCGTTA9550.10643574011847245No Hit
ATACATCACCTTACGGTTTAGCAGAGACCTGTGTTTTTGATAAACAGTCG9430.10509832767719321No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT9340.10409526834623378No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG9310.10376091523591398No Hit
GTTTAGCAGAGACCTGTGTTTTTGATAAACAGTCGCTTGGGCCTATTCAC9180.10231205175786147No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA9100.10142044346367532No Hit
GTTAAGAGGAGTGGTTAGCTTCTGCGAAGCTACGAATCGAAGCCCCAGTA9060.10097463931658224No Hit
CCTTAGAATTCTCATCTTGACTACCTGTGTCGGTTTGCGGTACGGGCACC9010.10041738413271589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2800.029.99947439
ACTGTGT853.9548268E-628.8262398
ATAGACC500.007206232328.002633
CTACACT500.007206232328.002634
ATATAAG3550.027.6082272
CGATTAG1056.774899E-726.66620314
GCCGTCT3300.026.51468746
CTAGGGG808.6630534E-526.2524663
GGTCCAA4600.025.86911211
TAGGGGT700.001225673625.0023484
GCTTGAA3500.024.99956156
CGTATGC3650.024.9310741
GTATGCC3550.024.64745742
TCTTACC1052.0384987E-523.3355278
TCTGGGG2550.023.3355268
CGCGTAA3900.023.33292421
GATCTGG15700.023.1868926
ACCGGTA4100.023.04837815
TGCCGTC3850.022.72687545
GACGTAA1854.0199666E-1022.7124311
Produced by FastQC (version 0.11.5)