FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575690
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT17030.2958189303270858No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC13300.23102711528774164No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC11050.19194358074658235No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC10730.1863850336118397No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT8980.15598672896871582RNA PCR Primer, Index 29 (95% over 23bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC8790.15268634160746236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT1900.027.637721
ATCTGGG5350.027.4851267
CTCGTAT3850.027.27167739
TAACTCT657.940136E-426.9313984
TATAGTA2250.026.4526182
AGTACTA2150.026.0545625
GTATGCC4250.025.52842942
ACTATAC700.001223604125.0077293
TGCTTGA4250.024.70493155
GTTTAGG1001.3947831E-524.5054441
CCGTCTT4200.024.16573747
TCTCGTA4500.024.11018438
GTCTTCT4100.023.9015249
CGTATGC4700.023.8288741
TTGAAAA4850.023.81351758
TTTCGGA2500.023.8052881
TATATGG901.9232137E-423.3405442
GTTCGTA901.9277485E-423.33243426
AAAGGGG4900.022.85626468
CTTGAAA4600.022.8252157
Produced by FastQC (version 0.11.5)