FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635479
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT9680.1523260406716823No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT9500.14949353165092788No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT7500.11802120919810095No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT7210.11345772244244105No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6740.10606172666602673No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA6720.10574700344149845No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA6420.10102615507357443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAG556.6880766E-638.187143
CCGATCT49900.035.846684
TCCGATC51900.035.0723273
TTCCGAT52300.034.8710172
CTTCCGA53950.033.7396431
CGATCTG24100.032.97145
ATCTGAG3300.030.7618667
GATCTGG12250.030.575696
TCTGAGC2050.029.0284378
GATCTAG3100.028.2297426
CGATCTA10400.026.5902445
ATCTGGG6200.025.4067697
ATCTTTT10000.025.2035127
TTACTCG700.001224955525.0034834
CATACAG700.001224955525.0034835
TAAGAGT851.3068826E-424.7093284
ATATTAG851.3068826E-424.7093281
GATCTGA9450.024.4478536
ATCTGAC3150.024.4478517
CGATCTT17950.024.3766575
Produced by FastQC (version 0.11.5)