FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454853
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT10350.22754604234774753No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT8110.17829936265123017No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6450.14180405537613253No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT6420.141144501630197No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA6360.139825394138326No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA6340.13938569164103567No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6060.133229856678971No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6010.13213060043574518No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5980.1314710466898097No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT5490.1206983355061965No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5370.11806012052245451No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5350.11762041802516417No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG4910.10794696308477683No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG4840.10640800434426068No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTA200.006949608752.49950835
TTCCGAT29100.036.0821382
CCGATCT28350.036.049044
CTTCCGA29700.035.4944531
TCCGATC29500.035.4742433
GTTGTAT400.00241019935.022771
ATCTTTT4950.031.1108197
CGATCTG15200.031.0852365
GGGTACA604.9798883E-429.16639116
TGTAGGG604.9798883E-429.1663912
ATCTGAG1950.028.7176787
TTTCGGA1352.564775E-1028.5370731
ATCTGTG1601.8189894E-1228.4372337
GATCTGG7550.028.277886
TCTTTTT5400.027.8701088
CTTTTTT5650.026.6369199
GATCTAG2250.026.4441956
TCTAACA700.001225482724.9997652
TCTATGC700.001225482724.9997658
TGGACAG1551.2860255E-924.8384785
Produced by FastQC (version 0.11.5)