FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833514
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC11080.13293118052006325No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA11000.13197138860295088No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC10070.12081380756651958No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT9220.11061601844720063No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT9110.10929630456117115No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT9100.10917633057153209No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG8870.10641692880983403No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT8840.1060570068409169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG36950.032.305535
CTCGTAT2650.031.69743539
TTCCGAT89800.030.6804242
CCGATCT89350.030.5196694
TCCGATC90900.030.3458443
GATCTGG18500.028.7613166
CTTCCGA95850.028.7090911
CTATAGA500.00720367328.0044384
GTATGCC3050.027.54039242
ATCTGGG9150.027.1611487
ATATGTG1850.026.4906865
TTTCGGA5050.025.650711
ATCTGGT4800.025.5233487
CGATCTA18350.025.1811055
CCGTCTT3700.024.59406947
TCTTTTT15650.024.37647413
ATCTTTT15600.024.23025112
TGCTTGA3500.023.99948555
GATCTAG6050.023.7227696
ACCTAGG1502.4769179E-823.33283452
Produced by FastQC (version 0.11.5)