Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adR3wtZT14s69.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 327162 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 958 | 0.29282129342649815 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 746 | 0.22802159174965309 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 732 | 0.2237423661672199 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 639 | 0.19531608194105674 | No Hit |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT | 448 | 0.13693521863786137 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT | 399 | 0.12195792909934527 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 388 | 0.1185956804274335 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG | 381 | 0.11645606763621692 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 342 | 0.1045353677994388 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA | 333 | 0.10178443706787463 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGTATT | 65 | 4.8570837E-7 | 37.691154 | 24 |
AACTAGT | 40 | 0.0024136526 | 35.00963 | 7 |
GTGATTA | 50 | 1.7081221E-4 | 34.998928 | 50 |
ACTATAC | 85 | 1.0584881E-7 | 32.95024 | 3 |
GTATGTA | 75 | 1.4878733E-6 | 32.66567 | 16 |
CCTAACA | 45 | 0.004298208 | 31.11967 | 3 |
ACTAGTA | 45 | 0.004298208 | 31.11967 | 8 |
TGACAGG | 45 | 0.0043046447 | 31.11016 | 10 |
GTTCTTA | 80 | 2.462075E-6 | 30.624062 | 40 |
TTTCGGA | 80 | 2.462075E-6 | 30.624062 | 1 |
GTATAGT | 155 | 1.8189894E-12 | 29.353941 | 1 |
TGAACTA | 60 | 4.9685815E-4 | 29.174694 | 5 |
CTTGTAT | 60 | 4.9774844E-4 | 29.165775 | 61 |
CACTCCG | 60 | 4.9774844E-4 | 29.165775 | 70 |
AATGACT | 60 | 4.9774844E-4 | 29.165775 | 37 |
TTTACCG | 85 | 3.948562E-6 | 28.822649 | 30 |
CTTTATG | 85 | 3.948562E-6 | 28.822649 | 10 |
TCTTTTT | 650 | 0.0 | 28.546316 | 8 |
ATCTTTT | 630 | 0.0 | 28.34113 | 7 |
CTTATAT | 75 | 5.577196E-5 | 27.999146 | 43 |