FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327162
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC9580.29282129342649815No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC7460.22802159174965309No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC7320.2237423661672199No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC6390.19531608194105674No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4480.13693521863786137No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT3990.12195792909934527No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA3880.1185956804274335Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3810.11645606763621692No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC3420.1045353677994388Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3330.10178443706787463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATT654.8570837E-737.69115424
AACTAGT400.002413652635.009637
GTGATTA501.7081221E-434.99892850
ACTATAC851.0584881E-732.950243
GTATGTA751.4878733E-632.6656716
CCTAACA450.00429820831.119673
ACTAGTA450.00429820831.119678
TGACAGG450.004304644731.1101610
GTTCTTA802.462075E-630.62406240
TTTCGGA802.462075E-630.6240621
GTATAGT1551.8189894E-1229.3539411
TGAACTA604.9685815E-429.1746945
CTTGTAT604.9774844E-429.16577561
CACTCCG604.9774844E-429.16577570
AATGACT604.9774844E-429.16577537
TTTACCG853.948562E-628.82264930
CTTTATG853.948562E-628.82264910
TCTTTTT6500.028.5463168
ATCTTTT6300.028.341137
CTTATAT755.577196E-527.99914643