FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524176
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC7860.14994963523701962No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT7750.14785110344617075No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC7240.13812154696132595No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC6350.12114251701718506No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC5500.10492658954244377Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT5380.10263728213424497No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAATA652.0969766E-532.318662
GTCTTCT2300.031.9551749
GTATAAG450.00429919831.121673
CTCGTAT2450.029.99873439
GTATGCC2350.029.78597342
CAATACT500.00719491528.0095064
GCGTGTA500.00720833427.9988169
CCGTCTT2550.027.44982147
TATGCTT1554.3655746E-1127.1059725
CAGGGGT657.9554913E-426.9219414
TATGCCG2700.025.9248343
TTCTATA959.3604285E-625.7982272
TCTCGTA2850.025.78838338
ATGCCGT2900.025.34375644
TCTTCTG3050.025.24483750
ATCTTTT7100.024.64684512
CTTGAAA2900.024.1369157
GCTTGAA3200.024.06148356
TCTTTTT7450.023.4889413
CATATAC750.001828093523.3412513
Produced by FastQC (version 0.11.5)