H3VNWAFXY_n01_adR3wtZT14s60.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3VNWAFXY_n01_adR3wtZT14s60.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637470
Sequences flagged as poor quality	0
Sequence length	76
%GC	56

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT	2876	0.451158485889532	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	1434	0.22495176243587933	Illumina Paired End PCR Primer 2 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	1419	0.22259871052755423	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	1335	0.20942161984093371	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	1167	0.1830674384676926	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	891	0.1397712833545108	No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	738	0.1157701538895948	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	644	0.10102436193075753	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGAG	65	9.969881E-9	43.097977	5
ACTTAGG	55	2.986153E-4	31.82874	1
ATAATAC	45	0.0042967475	31.126318	3
GCACTAT	45	0.0042967475	31.126318	6
TCGTATG	725	0.0	28.963764	40
GTCTTCT	690	0.0	28.911295	49
GTATGCC	745	0.0	28.655983	42
CTCGTAT	640	0.0	28.43578	39
TGCTTGA	750	0.0	27.998306	55
TATGCCG	780	0.0	27.370138	43
TATATGG	65	7.932743E-4	26.936237	2
CCGTCTT	755	0.0	26.885792	47
GCCGTCT	770	0.0	26.816559	46
TTCTGCT	795	0.0	26.413494	52
ATCTTTT	1160	0.0	26.26283	7
TCTTTTT	1090	0.0	26.021889	8
CTTGAAA	810	0.0	25.924358	57
CGTATGC	830	0.0	25.721336	41
TTGAAAA	850	0.0	25.527868	58
GCTTGAA	825	0.0	25.453005	56