FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542775
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC92070.5967817731036607No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC85040.5512145322551896No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC81830.5304078689374666No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC65210.42267991119897586No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC37200.24112394872875176Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA32460.21040009074557212Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC22260.14428545964252726No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG18530.12010824650386479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3150.031.1207661
CCTATAT450.004307158831.1137033
GTATTAA802.4633628E-630.6345021
TAGGATA657.956702E-426.9253224
CTTACTC657.956702E-426.9253223
GTATGCC4200.025.83297542
TTCGGAA4100.024.7589652
TATACGG1001.3979126E-524.5028362
GCTTGAA4450.024.38168356
GTCTTCT4450.024.38168349
TGCTTGA4550.023.84582355
GCCGTCT4550.023.84582346
GTACATG1352.3087159E-723.3405741
GTTAATA1052.0393873E-523.3360352
TAATATT1352.3137727E-723.3352784
GGTCCAA2900.022.93071611
GACACTC98800.022.91972270
CTCGTAT4500.022.55524339
GAGACGA10450.022.43987866
GTCCATA800.002662756521.8817881
Produced by FastQC (version 0.11.5)