H3VNWAFXY_n01_adR3wtZT14s59.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3VNWAFXY_n01_adR3wtZT14s59.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	396164
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT	1159	0.2925556082834382	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	867	0.2188487596046082	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	782	0.19739299885905837	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	774	0.19537363314183015	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	632	0.15952989166102927	Illumina Paired End PCR Primer 2 (95% over 22bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	587	0.14817095950162054	No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	584	0.14741369735765997	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	535	0.13504508233963713	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAC	25	2.379002E-4	56.048107	1
AGTAGAA	40	0.0024163285	35.003536	2
CTAGTGC	50	1.7074504E-4	35.003532	3
CATAACT	40	0.0024178254	34.999115	34
GCCGTCT	375	0.0	33.599148	46
CTCGTAT	320	0.0	32.811672	39
GTATGCC	365	0.0	32.601917	42
TTTCGGA	140	0.0	32.52792	1
TATGCCG	380	0.0	31.314997	43
TTAGGTC	45	0.004305618	31.110327	23
GCTTAAT	45	0.004305618	31.110327	22
AGTCTAT	45	0.004305618	31.110327	54
CTTGAAA	390	0.0	30.51205	57
GTCTTCT	405	0.0	30.24615	49
GCTTGAA	415	0.0	29.517328	56
TCGTATG	395	0.0	29.239769	40
TGAACGG	60	4.9755245E-4	29.169613	5
TCTTCTG	410	0.0	29.023659	50
TTCTGCT	435	0.0	28.16021	52
AAGTACT	50	0.0072012045	28.002827	4