FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210194
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT5740.2730810584507645No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT2610.12417100392970302No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAG150.00222048170.014996
TGTAGGG252.3840024E-456.0119972
AACGTTC200.006944827752.4987559
TGTCTTC200.006944827752.4987532
TCTTTGC200.006944827752.498758
GAATTGT200.006944827752.4987515
CCGTCTT350.001253575439.9990547
ATCTTTG350.001253575439.999057
CCACTTA459.177542E-538.88796264
GTACTGG601.2080798E-535.015831
TCTGCGC708.656625E-734.999178
ACACGAA400.002415234534.9991717
CAAGTAA400.002415234534.9991727
GTGGGTT400.002415234534.9991714
CTTCTGC400.002415234534.9991751
AATCGGA400.002415234534.9991719
CACTTAA501.7059785E-434.9991765
TGCCGTC400.002415234534.9991745
AAGTCAA400.002415234534.9991732
ATCTGGC1650.033.9385837
Produced by FastQC (version 0.11.5)