FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671505
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT41290.6148874543004147No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT19930.2967960030081682TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT9740.1450473190817641RNA PCR Primer, Index 7 (95% over 23bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT7040.10483913001392395No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGTA200.006943477352.5139662
GTCCATA751.4875186E-632.6753541
CTCGTAT9200.032.33592639
TTCCGAT75750.031.4737152
CTATTAT450.004301595531.1193871
CGATCTG30100.030.9384575
CCGATCT76350.030.7678414
GCCGTCT9350.030.69420846
TCCGATC78150.030.5071533
GATCTTT15500.030.4919826
GTATGCC9700.030.30831142
CGATCTT23050.030.0730745
TTTCGGA5150.029.9108681
GTCTTCT9500.029.84115249
TCTTCTG9750.029.7939250
CTTCCGA81050.029.3292061
CCCTAAT604.9743586E-429.1744251
TCGTATG10200.029.16573740
CTTGAAA10000.029.0490757
TCTTTTT15000.028.9388758
Produced by FastQC (version 0.11.5)