FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230312
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT14270.6195942894855674No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT8250.35820973288408764TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT3660.15891486331584978RNA PCR Primer, Index 7 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT200.00694510252.49983614
CCTAAGA451.6799186E-646.6665232
ATATTGC350.001253698439.99987851
ATCTTTG556.6760567E-638.18177
TTCGTTT501.7062787E-434.99989317
CAATTTT501.7062787E-434.99989334
TCTACAA400.002415469134.999898
TCACTCG400.002415469134.999899
TGGTCAT400.002415469134.9998910
TCTGTGC957.588824E-933.157798
TTTCGGA1950.032.3146061
CCTAGGG450.004301445631.1110152
ATTAATT450.004301445631.1110153
CTAAGAT450.004301445631.1110153
CACTCGG450.004301445631.11101510
GACTCTG450.004301445631.11101565
ATCTGTG1357.2759576E-1231.1110157
GTAAACA450.004301445631.11101544
TACAAGT450.004301445631.1110154
GTCTTCT3100.029.35474849