Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adR3wtZT14s50.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 230312 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT | 1427 | 0.6195942894855674 | No Hit |
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT | 825 | 0.35820973288408764 | TruSeq Adapter, Index 7 (95% over 21bp) |
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT | 366 | 0.15891486331584978 | RNA PCR Primer, Index 7 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTAGACT | 20 | 0.006945102 | 52.499836 | 14 |
CCTAAGA | 45 | 1.6799186E-6 | 46.666523 | 2 |
ATATTGC | 35 | 0.0012536984 | 39.999878 | 51 |
ATCTTTG | 55 | 6.6760567E-6 | 38.1817 | 7 |
TTCGTTT | 50 | 1.7062787E-4 | 34.999893 | 17 |
CAATTTT | 50 | 1.7062787E-4 | 34.999893 | 34 |
TCTACAA | 40 | 0.0024154691 | 34.99989 | 8 |
TCACTCG | 40 | 0.0024154691 | 34.99989 | 9 |
TGGTCAT | 40 | 0.0024154691 | 34.99989 | 10 |
TCTGTGC | 95 | 7.588824E-9 | 33.15779 | 8 |
TTTCGGA | 195 | 0.0 | 32.314606 | 1 |
CCTAGGG | 45 | 0.0043014456 | 31.111015 | 2 |
ATTAATT | 45 | 0.0043014456 | 31.111015 | 3 |
CTAAGAT | 45 | 0.0043014456 | 31.111015 | 3 |
CACTCGG | 45 | 0.0043014456 | 31.111015 | 10 |
GACTCTG | 45 | 0.0043014456 | 31.111015 | 65 |
ATCTGTG | 135 | 7.2759576E-12 | 31.111015 | 7 |
GTAAACA | 45 | 0.0043014456 | 31.111015 | 44 |
TACAAGT | 45 | 0.0043014456 | 31.111015 | 4 |
GTCTTCT | 310 | 0.0 | 29.354748 | 49 |