FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200919
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC77880.6485033545143345No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC71010.5912971649211979No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC69420.5780573044476772No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC55860.46514377739048185No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC29590.24639463610784743Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA27450.2285749496843667Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC19120.15921140393315453No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG15850.13198225692157423No Hit
GTGCAGGGAGACGTGTGCTCTTCCGATCTGTGCAGGGAGACGTGTGCTCT13900.1157446921898979Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCC350.001254197840.010334
TTTCGGA2250.038.902171
ACACCGT652.1003876E-532.3160326
GTCCTAT450.00430134431.1217351
AACTAGT450.00430134431.1217351
TATTTGC450.004309240331.11007569
TTCGGAA2900.030.182722
ACTAGTA500.00719848628.0095632
CGATTTA500.007211668427.99906714
TGCTTGA2500.026.59911355
GCTTTAA700.001224147425.008541
GGGTACA851.3093643E-424.7050616
AGCATAC750.001829440823.3413012
GGTACAC901.9293779E-423.33255617
AACGCAA750.001833434123.33255670
CTTGAAA3000.023.33255657
TTGAAAA3000.023.33255658
AAATGAA750.001833434123.33255655
GACACTC77300.023.04580370
GGACCGT2004.3655746E-1122.7558756
Produced by FastQC (version 0.11.5)