Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adR3wtZT14s49.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 452734 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT | 1477 | 0.3262401321747428 | No Hit |
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT | 775 | 0.1711821952846484 | TruSeq Adapter, Index 7 (95% over 21bp) |
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT | 600 | 0.13252815118811487 | No Hit |
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT | 600 | 0.13252815118811487 | No Hit |
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA | 532 | 0.11750829405346186 | No Hit |
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG | 474 | 0.10469723943861076 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATTAT | 35 | 0.0012538724 | 40.00707 | 1 |
CGATCTA | 1070 | 0.0 | 35.013924 | 5 |
CGATCTG | 2555 | 0.0 | 33.98612 | 5 |
TTCCGAT | 6140 | 0.0 | 33.81638 | 2 |
CCGATCT | 6135 | 0.0 | 33.501507 | 4 |
TCCGATC | 6250 | 0.0 | 33.165188 | 3 |
TCTGTCC | 75 | 1.4858524E-6 | 32.676052 | 8 |
CTTCCGA | 6410 | 0.0 | 32.384815 | 1 |
CTCGTAT | 415 | 0.0 | 32.046776 | 39 |
GATCTAG | 405 | 0.0 | 31.123487 | 6 |
AGTAATA | 45 | 0.004306661 | 31.109735 | 63 |
CTGTGCG | 80 | 2.4650944E-6 | 30.623646 | 9 |
TCTTCTG | 435 | 0.0 | 30.573362 | 50 |
GTATGCC | 435 | 0.0 | 30.573362 | 42 |
GATCTGG | 1300 | 0.0 | 30.43518 | 6 |
GCTTGAA | 455 | 0.0 | 29.229479 | 56 |
TCCTAGG | 60 | 4.967996E-4 | 29.178267 | 2 |
CATAAAG | 60 | 4.967996E-4 | 29.178267 | 3 |
TCTACTC | 60 | 4.9712113E-4 | 29.175045 | 8 |
TTCTAAT | 60 | 4.974428E-4 | 29.171822 | 1 |