FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328688
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC7090.21570607992990312No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC6020.18315241201382465No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5730.1743294552889062No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4660.14177578737282773No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC4380.13325707053497543Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT4250.1293019520031154No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4080.1241298739229908Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAA150.002223544869.9995411
ATGCACT404.5877885E-543.7497144
CTCGTAT701.9212166E-839.99973739
GCGTCTA350.001254501439.99973738
CTAGCGC400.002417005834.999779
ATCCTAC552.9836202E-431.827661
TAGTGCG450.00430416831.11090717
GTATGCC1103.1710442E-828.63617742
TACTAAG755.570582E-528.0040762
GTTAGAA500.007203226427.99981737
GTCTAGT500.007203226427.99981740
CACTATG500.007203226427.99981751
AGTACCA755.576362E-527.9998175
CGGACTA500.007203226427.99981761
GATCTTT7700.027.7270936
CCTACAC1154.8825314E-827.3911273
TCTTTTT6950.027.1940678
TTAGAAC657.947484E-426.92290138
CGTTATT1207.376548E-826.24982810
ACTGTCC808.6525804E-526.2498288