Basic Statistics
Measure | Value |
---|---|
Filename | H3VNWAFXY_n01_adR3wtZT14s45.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 328688 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 709 | 0.21570607992990312 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 602 | 0.18315241201382465 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 573 | 0.1743294552889062 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 466 | 0.14177578737282773 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 438 | 0.13325707053497543 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT | 425 | 0.1293019520031154 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 408 | 0.1241298739229908 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCATAAA | 15 | 0.0022235448 | 69.99954 | 11 |
ATGCACT | 40 | 4.5877885E-5 | 43.749714 | 4 |
CTCGTAT | 70 | 1.9212166E-8 | 39.999737 | 39 |
GCGTCTA | 35 | 0.0012545014 | 39.999737 | 38 |
CTAGCGC | 40 | 0.0024170058 | 34.99977 | 9 |
ATCCTAC | 55 | 2.9836202E-4 | 31.82766 | 1 |
TAGTGCG | 45 | 0.004304168 | 31.110907 | 17 |
GTATGCC | 110 | 3.1710442E-8 | 28.636177 | 42 |
TACTAAG | 75 | 5.570582E-5 | 28.004076 | 2 |
GTTAGAA | 50 | 0.0072032264 | 27.999817 | 37 |
GTCTAGT | 50 | 0.0072032264 | 27.999817 | 40 |
CACTATG | 50 | 0.0072032264 | 27.999817 | 51 |
AGTACCA | 75 | 5.576362E-5 | 27.999817 | 5 |
CGGACTA | 50 | 0.0072032264 | 27.999817 | 61 |
GATCTTT | 770 | 0.0 | 27.727093 | 6 |
CCTACAC | 115 | 4.8825314E-8 | 27.391127 | 3 |
TCTTTTT | 695 | 0.0 | 27.194067 | 8 |
TTAGAAC | 65 | 7.947484E-4 | 26.922901 | 38 |
CGTTATT | 120 | 7.376548E-8 | 26.249828 | 10 |
ACTGTCC | 80 | 8.6525804E-5 | 26.249828 | 8 |