FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442991
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT8790.19842389574506028No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT5020.11332058664848721RNA PCR Primer, Index 47 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAT350.001253699940.0083
ATTATGC652.0982476E-532.314153
TGATAAG450.004301419531.1173342
TAAATGA901.7515049E-731.1173344
GTATGCT450.004306175331.1103114
TTTCGGA1401.2732926E-1130.0061
TAAGAGT500.007198627628.0056024
GTACGAA500.007198627628.0056024
GTCCCAC1502.910383E-1127.99927751
GTCTTCT2700.027.2215249
CACGAGT1305.3933036E-926.92238246
AATAGTA657.952771E-426.92238249
TCGTATG2850.025.78880940
ATCTTTT10100.025.64290212
TCTATAC1101.0127751E-625.4596373
CTTTTTT10450.025.45388814
CCGTCTT3200.025.155647
ATCACTG700.001223936625.0055
TCTTTTT10500.024.99935713
TATAGAG851.3054723E-424.7108232
Produced by FastQC (version 0.11.5)