FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605993
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT16840.27789099874090956No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT14010.23119078933255005No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT9410.15528232174299042No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA9260.15280704562593958No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA8690.1434009963811463No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT8240.13597516802999374No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT7940.13102461579589203Illumina PCR Primer Index 11 (95% over 22bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG7910.13052956057248186No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA7270.11996838247306488No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC7270.11996838247306488No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT7180.11848321680283437No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC6980.11518284864676656No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG6850.11303760934532248No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT6620.10924218596584448No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA6220.10264144965370887No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG6170.1018163576146919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGAG2150.030.9370147
TATGCTT2950.030.8542235
CTCGTAT4750.030.20967139
ATCTGTG2350.029.7937687
CCGATCT54950.028.859834
GTCTCAA3050.028.68771412
CTTGTCT2950.028.473779
TTCCGAT56300.028.2921712
ATGTCTA3000.027.99920833
TCCGATC57050.027.9815943
CGATCTG24100.027.5994135
CTTCCGA58100.027.5361561
GTATGCC5500.027.3628642
ATATAAG2050.027.3230652
TACATCC657.944783E-426.9289823
CGTAAAT3000.026.83257523
GTCTTCT4900.026.42782249
GCTTGAA6250.026.31925656
TATGCCG5500.026.0901743
TGCTTGA6050.026.03232255
Produced by FastQC (version 0.11.5)