FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375826
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC47920.3482998576854922No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC43240.3142839283455902No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC42520.3090507084471437No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC32920.23927444313452428No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT22470.16332007099734994No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC21080.15321704924896026Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA19740.1434774455490738Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT18080.13141196633876667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATGT802.4672954E-630.6278294
ATTAGAA657.9558406E-426.9255663
GTATGCC6400.026.79642942
GCCGTCT6500.026.38417646
CTCGTAT6450.026.0460839
GACCGTT2450.025.7166617
TTAATAC700.001226016825.0023123
GCTAATG700.001226796324.99958670
GTCTTCT7250.024.6202849
ACCGTTA2450.024.2879588
TTAGGAC2750.024.1840533
CCGTCTT7100.024.15452847
TGCTTGA7050.023.82939155
GCTTGAA7050.023.82939156
ATGCCGT7500.023.33294744
ATATAAG2600.022.8892272
TTTCGGA4750.022.848371
ATCTTTT20100.022.81056812
GGTCCAA5550.022.70232611
TCTTTTT20250.022.46876113
Produced by FastQC (version 0.11.5)