FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158714
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT9300.5859596506924405No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT4170.262736746600804Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT1980.12475269982484218Illumina PCR Primer Index 11 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACC200.00694105652.545
GCTAGGA200.00694105652.511
CTTAGGT200.00694105652.523
CTAGGAG200.00694105652.512
GACGTGG352.0792748E-549.99999649
GAACATT305.8640144E-446.66666411
CTCTTCA350.001252501939.99999633
TATAGTT350.001252501939.9999965
GTATGCC1900.036.84210242
TATGCCG2050.035.8536643
TAGCCTG501.7038609E-435.00000456
TGCTTGA1900.035.055
TCTTAAC400.002413176235.03
TGCCGTC1900.035.045
CTTGAAA1950.034.10256657
GTCTTCT1650.033.93939249
CGTATGC2100.033.33333641
ATGCCGT2100.033.33333644
TTTCGGA957.556082E-933.1578941
CCGTCTT2050.032.43902647
Produced by FastQC (version 0.11.5)