H3VNWAFXY_n01_adR3wtZT14s38.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3VNWAFXY_n01_adR3wtZT14s38.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	588781
Sequences flagged as poor quality	0
Sequence length	76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT	1930	0.3277959037401003	No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA	1299	0.22062532588517633	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT	1288	0.21875705907629492	No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT	1226	0.20822682797169068	No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG	1212	0.20584903385129616	No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT	1162	0.19735691199274435	No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA	1145	0.1944695905608367	No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT	1135	0.19277116618912635	No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC	1061	0.18020282583846964	No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA	1052	0.17867424390393033	No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC	993	0.1686535401108392	No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG	976	0.16576621867893157	No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT	939	0.1594820485036032	No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG	896	0.15217882370524863	No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA	842	0.1430073320980127	No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA	829	0.14079938041478918	No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA	817	0.13876127116873677	No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT	800	0.13587394973682915	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	755	0.1282310400641325	No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA	746	0.1267024581295932	No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA	722	0.12262623963748831	No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC	689	0.1170214392108441	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	649	0.11022774172400264	No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC	648	0.1100578992868316	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	647	0.10988805684966058	Illumina PCR Primer Index 11 (95% over 22bp)
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA	644	0.10937852953814746	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA	640	0.10869915978946332	No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTT	640	0.10869915978946332	No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC	634	0.1076801051664371	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	617	0.10479278373452948	No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC	601	0.1020753047397929	No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGT	599	0.10173561986545082	No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT	595	0.10105625011676668	No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA	590	0.1002070379309115	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTATA	25	2.3901474E-4	55.999664	21
TATTATT	45	9.196759E-5	38.888653	2
CTCTAGG	40	0.0024135157	35.014656	1
CATCATA	40	0.0024185497	34.999786	22
CTCGTAT	330	0.0	32.87859	39
ACTGTCG	55	2.991802E-4	31.817987	8
AAAGGTG	80	2.466024E-6	30.624815	5
GTATGCC	375	0.0	29.866486	42
GTATTAA	60	4.9691804E-4	29.178879	1
TTACCTT	60	4.9815525E-4	29.166489	35
GTATTGG	110	3.1670425E-8	28.648354	1
ATCTTTT	855	0.0	28.245445	7
ATGCTAA	50	0.00720777	27.999832	11
TAGCACC	50	0.00720777	27.999832	4
AATTAGT	50	0.00720777	27.999832	31
AAGTCTT	50	0.00720777	27.999832	15
TCTTTTT	835	0.0	27.664501	8
CTTTTTT	825	0.0	27.57559	9
TATGCCG	395	0.0	27.468187	43
GATAGAC	65	7.954982E-4	26.922915	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content