FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605903
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT25990.4289465475496903No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT15080.24888472247207885Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT8160.1346750222395334Illumina PCR Primer Index 11 (95% over 24bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT7010.11569508650724622No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT6610.10909336973079849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG25650.029.6165965
TTCCGAT65550.029.3223152
CCGATCT65250.029.2400934
CTTCCGA67450.028.498691
TCCGATC67900.028.2535843
GATATAG755.5692082E-528.0107331
TCCTATA657.941004E-426.9311752
ATCTGAG2650.026.4186767
GATCTGG11800.026.1073886
CTCGTAT6300.025.55480436
GTATAAG700.001223149425.009581
ATCTGGT3800.024.8717927
ATCTTTT10000.024.49927712
TCTTCCG40500.024.0299325
GCTCTTC41000.023.5661143
GTATGCC6550.023.51075739
TGCTCTT41250.023.4252242
TCTGAGC1202.1635751E-623.3364968
GTGCTCT41400.023.2577041
TATGCCG6800.023.16108140
Produced by FastQC (version 0.11.5)