FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533729
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT19310.3617940940065089No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT10660.19972682765972993TruSeq Adapter, Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA350.001255197739.9997862
GCTTTAT350.001255197739.9997869
ATCTGTG2300.033.4780857
CTCGTAT4300.032.55796439
TTCCGAT54950.031.0826362
CGATCTG24550.030.6515675
CCGATCT55250.030.597124
GTATGCC4700.030.5317542
TCCGATC56750.030.343453
CTTCCGA56850.030.1782511
CTATAAG703.487505E-529.9998382
GTCTTCT4750.029.47352849
CCGTCTT5000.028.69984647
TCTTCTG4950.028.28267750
ATGATAC500.007207151527.9998537
TCTAACA500.007207151527.999852
TAGTGCG755.582747E-527.999854
CTTGAAA5200.027.59600457
GCCGTCT5250.027.33318746
GATCTGG10300.027.1843226
Produced by FastQC (version 0.11.5)