FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n01_adR3wtZT14s12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n01_adR3wtZT14s12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980930
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC58310.5944358924693913No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC54260.5531485427094696No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC51970.5298033498822546No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC42010.42826705269489157No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC18630.18992180889563984Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA17510.17850407266573556Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC14720.15006167616445618No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT13340.1359933940240384No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG11870.12100761522228906No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT501.7058325E-435.015581
TAATCAC400.002418820535.0012976
AATAGTG552.9924864E-431.8193635
TATAGGC500.00720317528.0053222
GTTAGAC808.667213E-526.2509733
GTATGCC4150.026.14421742
GTCTTCT4150.025.30085449
AGGACTA2250.024.8898145
CGTATGC4450.024.38168341
GACCGTT1301.6065496E-724.2316687
GCTTGAA4500.024.11077956
CTAGGAC2200.023.8645233
TGCTTGA4600.023.58662855
GCTCTAA750.001828100423.3437211
TATGCCG4650.023.33301243
CCGTCTT4500.023.33301247
CTCGTAT4700.023.08478739
CTTGAAA4550.023.07660557
GCCGTCT4550.023.07660546
GGTTGCA2600.022.88429816
Produced by FastQC (version 0.11.5)