FastQCFastQC Report
Sat 4 Aug 2018
H3VL5AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148688586
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2634250.17716558283767658No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2505710.1685206690982992No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2363640.1589657998361757No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2258450.15189128236110874No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG2084410.14018628168271102No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2031990.1366607925103276No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1912850.12864807255615437No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG1784390.120008539189417No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT1688690.11357226841877426No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC1611730.10839634993905989No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG1489340.10016505234638522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA2868000.044.320311
TTCCGAT2908300.043.698992
CCGATCT3096850.041.109464
TCCGATC4441500.028.836653
CGATCTG1865750.027.4402455
TATAGGT1431650.027.3545383
ATACTCC1247350.025.6599064
CCATACT1261250.025.1351932
TCCCCCC1217400.024.863518
CATACTC1369750.023.9967173
CGATCTA774200.023.634695
ATACCCG1594250.023.40975
CTAACGT1373900.022.9638883
CCTATAA1853650.022.85121
ACCTATA1604750.022.803481
CTATAAG1884800.022.4160022
ACCATAC1389650.022.3104081
AGGTCGC1849350.021.7564416
TACTCCC1596300.021.6720315
TATAAGC2000600.021.5356063
Produced by FastQC (version 0.11.5)