FastQCFastQC Report
Sat 4 Aug 2018
H3VL5AFXY_n01_p4H7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4H7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCACACGAACACTACCTTGCAAGGAGACTTCTTCAAGGGTAGTTTAA12.0No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG12.0No Hit
CATTAACATACAGAGAAATCAAACTATGTACCGGAAAAACATCGGTACTT12.0No Hit
CCTTGCGCCTTACGGGTTTACTCACCCGTTGACTCGCCCACATGTCAGAC12.0No Hit
AGTGACAATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAAT12.0No Hit
TTCCCCGGGCGTCGAACAGTCAGCTCAGAACTGGTACGGACAAGGGGAAT12.0No Hit
GTATACCTATCTCCCTAAGAACTATCAAATCAGGAGTTTGTCATGTCTCA12.0No Hit
CCCGGGGAGAGCTCCCGAGAGAGCCGTTCCCAGTCCGTCCCCCGGCCGGC12.0No Hit
CTACACTGATGTATTCAACGAGTTCACACCTTGGCCGACATGCCCGGGAA12.0No Hit
CCATCATTTAATCTTCCAGCACCGGGCAGGCGTCACCCCCTATACGTCCA12.0No Hit
GCTCGTAGTTGAACCTTGGGATGGGTCGGCTGGTCCGCCTTTGGTGTGCA12.0No Hit
TCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTC12.0No Hit
TCGCAATCCTATGTTTTAATTAAACAGTCTGATTCCCCTACTCCGTACCA12.0No Hit
GGGAGGTAGTGACAATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAA12.0No Hit
CCCTCCAATGGATCCTCGTTAAGGGATTTAGAATGTACTCATTCCAATTA12.0No Hit
CCAAAAAGCAACATCCGCAGATCCCTGGACGGCATCGTTTATGGATGAGA12.0No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG12.0No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG12.0No Hit
CAATGAAACGTGTCTCTCTCTTCACTCAATTTGTGAGTGAAGCAACAGCA12.0No Hit
GTTCAACTACGAGATTTTTAACTGCAAAAAATTAAAAATACGCTATTGGA12.0No Hit
CATAAGGTGCCAGCGGAGTCCTATAAGCCACATCCGCTGATCCCGGGTCG12.0No Hit
TAAGGTGCCAGCGGAGTCCTATAAGCAACATAAGCTGATCCCTGGTCGGC12.0No Hit
GTATAGGGGCGAAAGACTAATCGAACAATCTAGTAGCTGGTACAATACGA12.0No Hit
TCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGG12.0No Hit
AGATAGTACCGTACCTCGAGCTAGATCTGTATCTCAACAGCTCAAAACCG12.0No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC12.0No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT12.0No Hit
CTTTTAATATAACAACAACATAAAATCTAACGCAATCAGTTTCAAAGAGC12.0No Hit
CATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGAT12.0No Hit
CGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGACCCCAAC12.0No Hit
ACCAAGAACCCACAAAGGGTGTTTGTCGAGTAAGACAGCACGACCGTCGA12.0No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCA12.0No Hit
AACCGACACTGGTAGGCAGGGTGAGAATCCCAAGGCGCTTGAGAGAACTC12.0No Hit
CTATGAAATCTATGTCAAAGCTGTGATGAAAAAAGAAGCTCTGGCTGCAG12.0No Hit
ATCATCAACCATGTAAACTGTTACTTATGATACATCAGGCTACACCTAAA12.0No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC12.0No Hit
ATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAA12.0No Hit
GTCCGGACATCAACCTCCTGGACCACACGATGTCGATGATCAACGCAGAC12.0No Hit
GCATTTGATCCATTCTTCCCAATAATCCCATTGCCGCAAACGCTCCAGCC12.0No Hit
CACCGGAACCCAAAAACTTTGATTTCTGATAAGCTGACAGCGTAGTCCTA12.0No Hit
ATTGGAGCTCGAATTACCGCGGATGCTGGCCCCAGACATGCCAACCAATG12.0No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTTGCTGGTTCCCTCC12.0No Hit
GTGTAGGATAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTAATTTTAA12.0No Hit
CCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCG12.0No Hit
CTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATG12.0No Hit
GCCGACACGTCCTACCTAATTAAAGTTTGAGAATAGGTAGAGGGCGTTGA12.0No Hit
CCACCAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAG12.0No Hit
GGTAACTACTACTCGGATAACCGTAGTAATTTTTGAGCTAATACTTGCAA12.0No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACACCAGGACGGTGGTC12.0No Hit
GAATCATGATAACTCGACGGATCGCATGGCCTCTGTGCTGGAGACGCATC12.0No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTAT50.0145.07
AAATCTA50.0145.06
GAAATCT50.0145.05
ATCTATG50.0145.08
ATGAAAT50.0145.03
TATGAAA50.0145.02
TCTATGT50.0145.09
CTATGAA50.0145.01
TGAAATC50.0145.04
GGGGGGG150.048.333332145
Produced by FastQC (version 0.11.5)