FastQCFastQC Report
Sat 4 Aug 2018
H3VL5AFXY_n01_p4D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATATAGTTCATGTGTTCTACATGTTCATCTCTTGGTGGAACAAAATC11.4285714285714286No Hit
GGTCCATTCGTTCTAGAAGCCAGAAATCGGTTGGGAAAAATATATCAGCA11.4285714285714286No Hit
GTGGAGGATGGACAAAGATAAAATCCGGTCAAAGCGGCAGAATCACAATT11.4285714285714286No Hit
AACAAGGTGAGTTCCCTGGCTAGTTTCCACCGCCTTATCTCCACTCTCAA11.4285714285714286No Hit
GATTTTGGAATGGCTAGGATCTTTGGATTGGAGGTCACCGAAGGGCACAC11.4285714285714286No Hit
AGCATGTGGATTAATTATATAATACGAGCAAAACCTTACCAGACCTTGAC11.4285714285714286No Hit
GCAACTTCCTCCCCCATTTCACAAAGCCCTATATCCAGACACCTGTCGTA11.4285714285714286No Hit
AAAACAAATAGTAACAACAGAGAAGATAAGAGTTCAATAAGAGGACGATA11.4285714285714286No Hit
CTTCCATATCAACTGATCTAAAATCAAACACACCATCCTCTAGACGGAGA11.4285714285714286No Hit
GCTCTGTCACTCGCCGTGGTCACCCTTGCTGGTTCAGTGTTCCTCAGGGG11.4285714285714286No Hit
CTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGCCAG11.4285714285714286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4285714285714286No Hit
GACACGAGAATTGCTGCTAAAGGTCCTGCTAATGAATAAGTCCAAAAGAT11.4285714285714286No Hit
CTGGTACAAAGCAAACCACACGGATTTGAGAGCACAGATTGATAATGGTA11.4285714285714286No Hit
CATTTGTGCCCATCTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCC11.4285714285714286No Hit
CGCGAGTTATATCGGGTAAAGCCAATGATTAGAGGCATTTGGGGCGCAAC11.4285714285714286No Hit
CACCTAAACAAGAAGATCTCACAACACCTTTACCTGGTTGGATCATAGAG11.4285714285714286No Hit
GGACAATGAAGAGAAAGAGGATACCAAAATTCATATCAATTTGGATTTCA11.4285714285714286No Hit
TCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTT11.4285714285714286No Hit
TTCGTATGCCGACAATTTTTCTGGGTAGGTAGAACTATTGGAAGCAAATA11.4285714285714286No Hit
GATTTTCACGGTGGTTGAGCAAGAATCGAACCATAACGGACCGGTTTTCT11.4285714285714286No Hit
GTACTCTTCCATCTCATTAAGCCAAAGATCCGCAAACTCGCAATCCTTCC11.4285714285714286No Hit
CTTTTGGAGTTGATTTCCAGTTTTATTCCACTGTGGTTTGAAGAGTGCTT11.4285714285714286No Hit
CTAGAAAGCTCAAAGGATACTCAAGAAGATGGACCGAGAAAAAATGAATC11.4285714285714286No Hit
GGTAGTAAGTAGTATAGCTGTCCAGGTATGATGCCAAGAAGATCTGGCAT11.4285714285714286No Hit
GAAATATATTCCACATTCTCAAACCAACAATCGACTAAACACGAAAAATA11.4285714285714286No Hit
GATACATTAATACGACAAATATTTCTATGATAATTTCTAATAAAGTCACG11.4285714285714286No Hit
GACCTGAAGATGAGAATCATGGAGTAGAGGTCTTGCCGTTTCCTATGTTC11.4285714285714286No Hit
ACGTGGGAAGGGTAAAATCGGGAATTATAGTTTTACAAAGAGGATGAGAG11.4285714285714286No Hit
GTTCAACATAGAATCAATGATAACTCAAATTAAAGAGCTTACAAAATTCC11.4285714285714286No Hit
GTTCTATCCAATAGAAATTAAACAAGGATATAACTAATAAATAATATGAA11.4285714285714286No Hit
TCCGTCTACAGAATGGATTCTCAAATCAAACACGCAGTGGTCGTGAAGGT11.4285714285714286No Hit
CTCGTAGACAGAGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC11.4285714285714286No Hit
GTGGAGTACTGTGGTGAAGTAATATCATGGAAAGAAGCAAAGAAGAGGGC11.4285714285714286No Hit
GGGATAGAGAAAGGGAAACTTCAAGTAGTGTCGGAAGGAAAATAATTCAT11.4285714285714286No Hit
GGAGACACGGTTCAAGAAACAAGAATTTATAATTTGCTGAGAATGATTCT11.4285714285714286No Hit
GAGAAGGATTTCCCGCTAAGCAGCAGTTCTTCCTTACCAACTTAGCTGCC11.4285714285714286No Hit
GATGTGGAAGAGTTCATATTCAAGGACTTTGAGGGTGAGAGGAATTGAAG11.4285714285714286No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC11.4285714285714286No Hit
GTATTGGTGGCGCTTTGCCTCCTAAGAAGGATCTTTCTCGTTACATCAAA11.4285714285714286No Hit
GCTTAAACTAGCACTCTCTTTCTATATACCTATTACATTTTTCTTTCTAA11.4285714285714286No Hit
GTGTGGTATCATAGTCAAAGCACCAGTATCCTTGGAGAAATCAAGTTGTA11.4285714285714286No Hit
ATCATAGATCAAACCATATCCAGAAGATTTACAACCTCCAAAGTGAGTTC11.4285714285714286No Hit
TCCCTTCGCATGTCAAGTGACTCGAGCATATTATCACACTCCAAAAGAAT11.4285714285714286No Hit
ATATGAAGGTGATAGACAGGATAAAAGTGGAAGCTTCATAGTCTTGGAAA11.4285714285714286No Hit
CTCTTTAAGTCCTTGCCTAAACATGTCATTCTCCTGAATATTCACATCTT11.4285714285714286No Hit
AAACATACACGATCACTAACAGAGATCAGCTGGGGAAACCAGATACGAAC11.4285714285714286No Hit
GTCCTGGTTCTCCCAAATCTGTGCGAATCGTTGTGGTTGGTGATAAGGGC11.4285714285714286No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGC11.4285714285714286No Hit
GTCCACTAAACCTTATCATTTAGATGAAGGAGAAATCGTAACAAGGTTTC11.4285714285714286No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA11.4285714285714286No Hit
GGCCTACCATGGTGGTAACGGGTGACGGAGATTTAGGGTTCGATTCCGGA11.4285714285714286No Hit
TAGCTAGTGTTCGTAGGCTCCATGCTATCGCATCGAACGACCTACCACGT11.4285714285714286No Hit
ATTTATAAGTTAACTTGTTTCATGAGGAGAATATTTATGACAAGAAGTAA11.4285714285714286No Hit
GCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCT11.4285714285714286No Hit
GTCGTAGGCAACAGGAACCGTGAAGAGAAACACAAGAGCTATGTATGACG11.4285714285714286No Hit
TATCATAACCGAACAGAAGGCCACCGATTCCGGCAGGGACCGTCAAACCC11.4285714285714286No Hit
GCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGAC11.4285714285714286No Hit
CTCCCAGACTATTCAGAACAGCGGGAACCAATTTGATGCTCATGGAAACA11.4285714285714286No Hit
GGGAACACCATCTTGGTACCTGCTAAGTACTTCTTTGGTATGAGCAAGTA11.4285714285714286No Hit
GAATCATCTGGGAGATTGACAAAGAAGAGTTTAAGACAGTGATGAGTCTG11.4285714285714286No Hit
GGATTAACATAAATAACAATGAGAAGTGTATTATCAAGATTCTCAAGCCT11.4285714285714286No Hit
CCAGTCGTCACCACCATGCCAGCACCACATTCGGTAGATGATCACAAACC11.4285714285714286No Hit
CAAACGGTGTTGTCATGTCCTGAGAGTCCAAAAATTTCCATCTTCGTACC11.4285714285714286No Hit
GCTTAAACTCAATCGGGATTACCCGATGAGTTTAATAATATCAATAATCT11.4285714285714286No Hit
TCAATCATCTTGTGCAAGATGAACAGCCTGAGCAAATGGTCGATAATTCA11.4285714285714286No Hit
GGAAAAAGATGACGGTCAAGACCTCGTAATTTCTCTCTTTCCATTGCGTA11.4285714285714286No Hit
CAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGA11.4285714285714286No Hit
GTTCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTACTTGG11.4285714285714286No Hit
GAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACCAAGACGCGCTG11.4285714285714286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAC50.0145.0145
GCCCATC50.0145.08
CATTTGT50.0145.01
CCCATCT50.0145.09
TGCCCAT50.0145.07
ATTTGTG50.0145.02
TTGTGCC50.0145.04
TTTGTGC50.0145.03
TGTGCCC50.0145.05
GTGCCCA50.0145.06
Produced by FastQC (version 0.11.5)