FastQCFastQC Report
Sat 4 Aug 2018
H3VL5AFXY_n01_p4B7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4B7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23.076923076923077No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG23.076923076923077No Hit
CTAAACGGCCATAGTCCCTCTAAGACGCCGGCCGTGAATGGATGCATCCA11.5384615384615385No Hit
AATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTG11.5384615384615385No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC11.5384615384615385No Hit
GTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCACA11.5384615384615385No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG11.5384615384615385No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11.5384615384615385No Hit
ATGCCTGAGCGGGGTAAAGCCAGAGGAAACACTGGTGGAAGCCCGCAGCG11.5384615384615385No Hit
GTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGC11.5384615384615385No Hit
GTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTA11.5384615384615385No Hit
CATCAACAGCGCTGAAACCGCTTTAGTTAGGCGTCCGATTAGGGATGAAC11.5384615384615385No Hit
AGCGGATGGGGGCCGGAGATGCGTAATGGTCGGATGAGGAACGGAGCAAT11.5384615384615385No Hit
ATAACGGGTAAAGCCAATGATTAGACGCATAGCTGTCGCAACTCCCTAGA11.5384615384615385No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT11.5384615384615385No Hit
AGCCTACGCTATGGATACATTAGCATGGGATAACATCATAGGATTTCGAT11.5384615384615385No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTA11.5384615384615385No Hit
ACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCACTCTTCTGC11.5384615384615385No Hit
GGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACG11.5384615384615385No Hit
GATTATGGTTGAGACTAGGACGGTATCACATCGTCTTCGAGCCCCCAACA11.5384615384615385No Hit
CTAAATACATCGGCTCCATCCGCTTCCACTATAACGGTTTCACGTTCTCT11.5384615384615385No Hit
GCCGTGATCGTGGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAG11.5384615384615385No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG11.5384615384615385No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGTTTCGATTAGT11.5384615384615385No Hit
CGCTTCCGCCGAACGGACTTAGCCAACGACACGTGCCTTTGGGAGCCTAA11.5384615384615385No Hit
GCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGA11.5384615384615385No Hit
GTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCA11.5384615384615385No Hit
CCCTTGGGACCTTATTCAACCCCAGGATGTGATGAGTCGACATCGAGGTG11.5384615384615385No Hit
CGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCACCAAC11.5384615384615385No Hit
GCCATGGTGCGCTAGAAGAAGATGCCGGTCCTGACCAGGAGGTCGTCATC11.5384615384615385No Hit
AAGTTTCCCTCAGGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAA11.5384615384615385No Hit
CCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTA11.5384615384615385No Hit
TATTTGCTACTCCCACCAAGATCTGCCCCGACGGCCGCTCCGCCCGGGCT11.5384615384615385No Hit
TGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTC11.5384615384615385No Hit
CCCCGAAAATGGATGGCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGG11.5384615384615385No Hit
AAAAAGAAGTGAACACTTTGGAACGAAGCTTCGTCTTTCTTTTTCTTAAA11.5384615384615385No Hit
GACTAACCCATGTGCAACTGCCGTTCACAAGCAACCTTTCCACTCATCGG11.5384615384615385No Hit
GACCAGGACGCATCGCCGGCCCCCAGCCGCTTCCCTACCGACAATTTCAA11.5384615384615385No Hit
GAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCG11.5384615384615385No Hit
GCAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACC11.5384615384615385No Hit
CAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATACAGAGC11.5384615384615385No Hit
ATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTAAAAG11.5384615384615385No Hit
CTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGT11.5384615384615385No Hit
GATCTAACATGGGCAAGCAGCAGCAAGTAAAGAAAGCAAGCTAGACCTTA11.5384615384615385No Hit
CTACCTAGCATAACAGACTAGGACAAATAAAAAAGTATAAGACTCAGGAG11.5384615384615385No Hit
GCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGTGTTC11.5384615384615385No Hit
CATCTGTAATCCTCCAGAAGCCGGAAACTCTCCAGGTTCTCAACCAACAT11.5384615384615385No Hit
TCCAATGCTAATGTATCCAGAGAGTAGGCTTGCTTTGATCACTCTAATTT11.5384615384615385No Hit
ATTCTAAAAATTAAAATAGTTAGGACGTGTAGTCTGATATGTAGACCCTT11.5384615384615385No Hit
CCCAGGATGTCGACATGTCGTATTACCCCAAGGCCTCAGCCTCCACCGGC11.5384615384615385No Hit
GGAAAAACGTAATCCTGCAGCACTGCCAAGATTGTCTCAACTTCACATTT11.5384615384615385No Hit
ATATATGCCATATATTTTTGTGTATGTTACCAAGGCCGTTGTACATATCA11.5384615384615385No Hit
GTGCTCTTACGATATTCCATGGGGAGTCTGGTAATTGGAATGAGTACAAT11.5384615384615385No Hit
TTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGC11.5384615384615385No Hit
GTTGAATACATAGTCGTATGAGGCGAACCGGGGGAACTGAAACATCTAAG11.5384615384615385No Hit
TTTCACCTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTGTTAATC11.5384615384615385No Hit
TGATTAATGAAAACATACTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTC11.5384615384615385No Hit
CTACGGTGCTGAAGTGATAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGC11.5384615384615385No Hit
CTAAAGCTCATGGTGGTGTATCCGGATTTGGTGGAGTAGGCGAACTGACT11.5384615384615385No Hit
CACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTC11.5384615384615385No Hit
GACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGACGGGACC11.5384615384615385No Hit
CTTTCGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGG11.5384615384615385No Hit
ACCGCAGGCTAGACCACGACGGGCTCGGGCTCCAAACGAATACCGAAACG11.5384615384615385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGTC50.0145.08
CCAGCGG50.0145.04
GCGGAGT50.0145.07
GCCAGCG50.0145.03
AGCGGAG50.0145.06
TGCCAGC50.0145.02
TTGGTCG50.0145.0145
CAGCGGA50.0145.05
GGAGTCC50.0145.09
GTGCCAG50.0145.01
Produced by FastQC (version 0.11.5)