FastQCFastQC Report
Fri 13 Jul 2018
H3TN2AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3TN2AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5727127
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2142013.7401126254053736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGA2500.018.89715217
CCTCGGG2100.018.21138215
CTCGGTG4200.017.1368338
AGCGTCG5950.015.12469611
AGATCGG6600.015.0362321
TGTAGGG6650.014.88484518
CCCAGGC3100.014.51242134
GTGTAGG6550.014.08184717
CTCGGGG2905.456968E-1213.96316916
GTAGGGA6900.013.36731719
TCGTGTA7300.013.25131315
GATCGGA7550.013.1359712
GATCTCG4700.012.92200435
TCTCGGT5350.012.61271737
GTCTCCC3800.012.42853642
CAGGCGG3651.8189894E-1212.325294536
CCAGGCG3501.0913936E-1112.21095135
CGGTCTC3900.012.11038540
GAGTGTA7700.011.6855328
TCGGTGG7300.011.39970339
Produced by FastQC (version 0.11.5)